bcrp - publications

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1. Curr Drug Metab. 2012 May 16. [Epub ahead of print]

The SmartAmp method: Rapid Detection of SNPs in Thiopurine S-Methyltransferase
and ABC Transporters ABCC4 and ABCG2.

Aw W, Lezhava A, Andoh A, Tanaka H, Hayashizaki Y, Ishikawa T.

RIKEN Omics Science Center, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama 230-0045,
Japan. toshi-i@gsc.riken.jp.

Genetic polymorphisms of drug transporters as well as drug metabolizing enzymes
have been documented to play a significant role in patients´┐Ż responses to
medication. A key requirement for advancing personalized medicine is the ability
to rapidly and conveniently test for patients´┐Ż genetic polymorphisms. We have
recently developed a rapid and cost-effective method for single nucleotide
polymorphism (SNP) detection, named Smart Amplification Process (SmartAmp), which
enables us to detect genetic polymorphisms or mutations in 30 to 45 min under
isothermal conditions without the need for DNA isolation and PCR amplification.
This article presents the SmartAmp-based detection of SNPs in the thiopurine
S-methyltransferase gene as well as in the ATP-binding cassette (ABC) transporter
ABCC4 and ABCG2 genes that are critically involved in drug-induced adverse
reactions. The SmartAmp method is expected to provide a practical and
cost-effective tool for pharmacogenomics-based personalized medicine.

PMID: 22591349 [PubMed - as supplied by publisher]