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1. Oncol Rep. 2012 Apr;27(4):1177-87. doi: 10.3892/or.2011.1618. Epub 2011 Dec 30.

Identification of ABCG2+ cells in nasopharyngeal carcinoma cells.

Zhang H, Liu W, Feng X, Wang L, Jiang X, Liu D, Zhang L, Zhu B, Zhou W, Jia W, Li
G, Ren C.

Cancer Research Institute, Xiang-Ya School of Medicine, Central South University,
Key Laboratory for Carcinogenesis of Chinese Ministry of Health, Key Laboratory
for Carcinogenesis and Cancer Invasion of Chinese Ministry of Education,
Changsha, Hunan 410078, P.R. China.

Tumor stem cells are a small subset of tumor cells with the ability of
self-renewal and differentiation and are regarded as a cause of tumor growth and
recurrence. Previously we have shown that stem-like label-retaining cells (LRCs)
can be detected in nasopharynx, tongue, esophagus and xenograft tumors formed by
nasopharyngeal carcinoma (NPC) cell lines (5-8F, 6-10B and TMNE). The present
study aimed to identify ABCG2+ cells in 5-8F NPC cells and compare their
tumorigenic potential with ABCG2- cells, expecting that we can obtain insight
into the mechanism of the differential phenotypes of ABCG2+ and ABCG2- cells. By
using magnetic cell sorting (MACS) method, we isolated ABCG2+ cells and ABCG2-
cells from 5-8F cells. Among these two subpopulations and unsorted 5-8F cells,
the rate of ABCG2+ cells at G1 phase was highest, while the rate of ABCG2- cells
at S phase was highest, indicating that ABCG2+ cells were mostly quiescent.
However, ABCG2+ cells showed lower cloning efficiency and tumorigenicity than
ABCG2- cells. We also used Affymetrix U133 plus 2.0 human whole genome expression
chip to identify the gene expression profile of ABCG2+ and ABCG2- cells and found
that both subpopulations expressed some stem cell associated genes, e.g., PSCA,
ABCG2 and ALPI were expressed in ABCG2+ cells, and K19, integrin α6, integrin β4,
CD44 and K14 were expressed in ABCG2- cells, suggesting there were stem cells in
both ABCG2+ and ABCG2- cells. Our data demonstrated that there exist ABCG2+ cells
in NPC cells, but ABCG2 alone is not sufficient for isolating cancer stem cells
in 5-8F NPC cells.

PMID: 22209971 [PubMed - in process]