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1. Gastric Cancer. 2012 Feb 14. [Epub ahead of print]

Expressions of putative cancer stem cell markers ABCB1, ABCG2, and CD133 are
correlated with the degree of differentiation of gastric cancer.

Jiang Y, He Y, Li H, Li HN, Zhang L, Hu W, Sun YM, Chen FL, Jin XM.

Department of Pathology, Harbin Medical University, 157 Xuefu Road, Nangang
District, Harbin, 150081, Heilongjiang, People's Republic of China.

BACKGROUND: The present study was carried out to determine whether a quantitative
relationship exists between the expressions of 3 cancer stem cell (CSC) markers
and the degree of differentiation of gastric cancer. METHODS: The expressions of
3 putative CSC markers, ABCB1, ABCG2, and CD133, were detected in 90 human
gastric adenocarcinoma cases by immunofluorescence assay. The differentiation
statuses of 3 gastric cancer cell lines (the undifferentiated gastric cancer cell
line HGC-27, the poorly differentiated gastric cancer cell line BGC-823, and the
moderately-poorly differentiated gastric adenocarcinoma cell line SGC-7901) were
observed and compared by performing the 3-[4,5-dimethylthiazol-2yl]-2,5-diphenyl
tetrazolium bromide (MTT) assay. Gastric xenotransplant cancers in nude mice were
constructed to compare the malignancy of the 3 variously differentiated gastric
cancer cell lines. The expressions of the 3 putative CSC markers were also
detected in the 3 gastric cancer cell lines in vitro by flow cytometric analysis
and in the 3 gastric xenotransplant cancers in vivo by immunofluorescence
staining. RESULTS: The expressions of ABCB1, ABCG2, and CD133 were generally
correlated with the degree of differentiation of the gastric cancers. In the
human gastric adenocarcinomas and in the cancer cell lines, the expressions of
ABCB1, ABCG2, and CD133 increased with the increases in the malignancy grades of
the gastric cancers. In the human gastric adenocarcinomas, poorly differentiated
adenocarcinoma expressed more ABCB1, ABCG2, and CD133 than well-differentiated
adenocarcinoma. In addition, the expressions of ABCB1 and CD133 were higher in
the diffuse type than in the intestinal type of human gastric cancers. The
undifferentiated cell line HGC-27 expressed more putative CSC markers than the
moderately-poorly differentiated cell line SGC-7901. Similar results were
observed in the xenotransplant tumors that arose from the 3 gastric cancer cell
lines. CONCLUSIONS: The expressions of the CSC markers ABCB1, ABCG2, and CD133
differed in the gastric cancers with various degrees of differentiation, with
poorly differentiated gastric cancer expressing relatively more CSC markers.

PMID: 22395309 [PubMed - as supplied by publisher]