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1. Cancer Lett. 2012 Apr 17. [Epub ahead of print]

Breast cancer, side population cells and ABCG2 expression.

Britton KM, Eyre R, Harvey IJ, Stemke-Hale K, Browell D, Lennard TW, Meeson AP.

Institute of Genetic Medicine, Newcastle University, Central Parkway,
Newcastle-upon-Tyne NE1 3BZ, UK.

Recurrent metastatic breast cancer may arise in part due to the presence of drug
resistant adult stem cells such as Side Population (SP) cells, whose phenotype
has been demonstrated to be due to the expression of ABCG2. We hypothesised that
SP may be identified in Fine Needle Aspirates (FNAs) and their presence may be
determined by expression of ABCG2 in breast tumours. SP and non-side population
cells (NSP) were isolated using dual wavelength flow cytometry combined with
Hoechst 33342 dye efflux and analysed for expression of ABCG2 and
chemoresistance. FNA samples used in SP analysis were matched with
paraffin-embedded tissue which was used in immunohistochemical analysis to assess
ABCG2 expression. Results were correlated to the pathobiology of the tumour. MCF7
and MDA-MB-231 cell lines contain SP cells. MCF7 SP have increased expression of
ABCG2 and increased resistance to mitoxantrone compared to NSP cells. The
presence of SP in FNAs were significantly associated with ER-negative (p=0.008)
and with triple negative breast cancers (p=0.011) which were also found to have a
significant increase in ABCG2 protein expression. ABCG2 transcript was detected
in some but not all SP cell populations isolated from FNA samples.

Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.

PMID: 22521545 [PubMed - as supplied by publisher]