bcrp - publications

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1. Chin J Cancer. 2012 Mar;31(3):150-8. doi: 10.5732/cjc.011.10310. Epub 2012 Feb

ABCG2-overexpressing S1-M1-80 cell xenografts in nude mice keep original
biochemistry and cell biological properties.

Wang F, Liang YJ, Wu XP, Su XD, Fu LW.

State Key Laboratory of Oncology in South China, Sun Yat-sen University Cancer
Center, Guangzhou, Guangdong 510060, P. R. China. fulw@mail.sysu.edu.cn.

S1-M1-80 cells, derived from human colon carcinoma S1 cells, are
mitoxantrone-selected ABCG2-overexpressing cells and are widely used in in vitro
studies of multidrug resistance(MDR). In this study, S1-M1-80 cell xenografts
were established to investigate whether the MDR phenotype and cell biological
properties were maintained in vivo. Our results showed that the proliferation,
cell cycle, and ABCG2 expression level in S1-M1-80 cells were similar to those in
cells isolated from S1-M1-80 cell xenografts (named xS1-M1-80 cells).
Consistently, xS1-M1-80 cells exhibited high levels of resistance to ABCG2
substrates such as mitoxantrone and topotecan, but remained sensitive to the
non-ABCG2 substrate cisplatin. Furthermore, the specific ABCG2 inhibitor Ko143
potently sensitized xS1-M1-80 cells to mitoxantrone and topotecan. These results
suggest that S1-M1-80 cell xenografts in nude mice retain their original
cytological characteristics at 9 weeks. Thus, this model could serve as a good
system for further investigation of ABCG2-mediated MDR.

PMID: 22360854 [PubMed - in process]